Transcription factor ZmPLATZ2 positively regulate the starch synthesis in maize

Abstract Maize is one of the three major crops worldwide based on its yield and quality. Starch crucial to both quality maize as it accounts more than 60% seed weight, structure influences crop. synthase I (SSI) contributes majority starch activity in endosperm. An in-depth understanding synthesis regulatory mechanism would provide opportunities for improving maize. In this study, ZmPLATZ2 , a plant AT-rich sequence zinc-binding protein (PLATZ) transcription factor related synthesis, was selected co-expression analysis. The semiquantitative RT-PCR qRT-PCR assays revealed that had high expression endosperm, reached peak at 12 days after pollination (DAP). Different treatments demonstrated downregulated by presence sucrose. Subsequent transactivation subcellular localization analyses showed localized nuclei without transactivation. Yeast one-hybrid transient endosperm indicated could bind promoters ZmSSI ZmISA1 ZmISA2 increase their gene expression. After overexpression rice, four genes were significantly upregulated transgenic plant, including OsSSI gene. vitro DAP-seq data CAAAAAAA element. conclusion, our support binds element promoter mediates Glu signal pathway.